Ototoxicity was investigated in male and female Hartley Guinea Pigs treated with 14 consecutive days of gentamicin at 40, 80, or 160 mg/kg given subcutaneously and compared to saline-treated controls. Guinea pigs are a common model of ototoxicity, but no sex differences have previously been reported. Baseline auditory brainstem response (ABR) tests were performed before the first dose and again 14 days after the last dose of gentamicin or saline. Cochlea were dissected using a whole mount preparation and immunofluorescent staining was performed using myosin VIIa and the nuclear dye, Hoeschst.
Presented in conjunction with Turner Scientific