People with type I diabetes (T1D) can have renal complications such as hyperfiltration and vascular restructuring that can potentially result in renal failure. The objective of this study was to determine if the induction of T1D in miniature swine impacted renal function by evaluating the glomerular filtration rate (GFR) and renal blood flow (RBF). Yucatan males (>6 months old) were chemically induced into a diabetic state using alloxan. Three diabetic and three control animals were fasted overnight then administrated iodohippurate (15mg/kg) and Iohexol (1.25mg/kg), independently, via an intravenous injection. Blood samples were obtained prior to dosing and at 6 time points following dosing to evaluate the clearance rate of either iodohippurate (RBF) or iohexol (GFR). In the diabetic miniature swine, there was a 48% increase in GFR and a 43% increase in RBF, compared to control swine. The GFR increase suggests there may be a hyperfiltration phenotype in the alloxan-induced diabetic miniature swine, similar to what has been noted in patients with T1D. Furthermore, the increase in renal blood flow also suggests there may be some vascular restructuring occurring in the animal model. While further investigation into the correlation between renal dysfunction and diabetes will be needed, these preliminary data suggest the alloxan-induced diabetic miniature pig may be a good translational model for T1D renal function research.
Walters, E.M., Boley, S.E., Brocksmith, D.F., Bouchard, G.F.