Experimental Induction of Allergic Contact Dermatitis in Sinclair, Hanford, and Yucatan Minipigs
Stricker-Krongrad, A., Jones, M.R., Bouchard, G.F.
The objectives of this study were to induce and characterize allergic contact dermatitis using 2,4-dinitrofluorobenzene (DNFB) in Sinclair, Yucatan, and Hanford minipigs, and assess the efficacy of corticosteroids in treatment of dermatitis (N = 3 per breed, all males). During acclimation the entire dorsum of the minipig was shaved, washed, and topical dermal challenge sites were marked on the dorsum of each animal. Sensitization Phase: On Day 1, animals were topically sensitized with 10% DNFB distributed among the left and right ears, and left and right medial thighs. Challenge Phase: On Study Days 14, animals were challenged on designated dose sites with 1% DNFB. Dose administration: Approximately 2-4 hours post challenge, dose sites on the right side of the animal were treated with 1% hydrocortisone. Dose Site Scoring: Dose sites were scored for erythema and edema using three point grading scales (maximum total score: 6) during the course of three days post-challenge. Histopathology: Dose sites from one animal of each breed of minipig were
processed to slides, stained with H&E, and evaluated by a pathologist for signs of dermatitis (24 hours post-challenge).