Electroretinography (ERG) of C57BL/6 and NIH III Mice

Authors

Leigh, H., Blair, E., Liu, J., Brown, L., Bouchard, G.F.

Abstract

Electroretinography(ERG) is a unique tool for measuring the electrical potentials of the retina and evaluating in-development drug products for potential ocular effects, such as induced retinal degeneration. The mouse has become a popular model for the study of retinal degeneration, and new generation electrodes have been used successfully to obtain electroretinogramsfrom mice as young as 14 days. The C57BL/6 mouse (female, 7-8 weeks, 13-17g) and NIH III strain of nude mouse (male, aged 6-8 weeks, 19-28g) were used in this baseline study of niaveanimals. We measured ERGs of 5 C57BL/6 and 59 NIH III untreated mice using the DiagnosysLLC, EspionE² System. All C57BL/6 mice had acceptable ERG recordings while 12 (20%) of the NIH III mice had flat-line recordings, which might be a result of retinal degeneration. Mean values for retinal ERG potentials of the NIH III and C57BL/6 are presented below. These data offer baseline ERG measurements for the NIH III and C57BL/6 strains of mice.

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