Blood Glucose (bG) Profiles Following IVGTT in Two Miniature Swine Lineages

Authors

Lawson, C., Leigh, H., Liu, J., Brown, L., Bouchard, G.F.

Abstract

Diabetes mellitus is an epidemic which is increasing in prevalence worldwide, especially in children. Two main types of diabetes are recognized: Type 1 (juvenile onset or insulin-dependent) and Type 2 (adult onset or non-insulin-dependent) and miniature swine is becoming an increasingly recognized model. A number of standard tests are commonly used to monitor the severity of diabetes in lab animals, for instance, OGTT, IVGTT, glucose clamps, insulin resistance, glucose intolerance, hypertension, dyslipidemia, ERG, histology [e.g. atherosclerosis]. Some tests still lack some validation in the miniature swine. Hypothesis: a glucose dose of 600 mg/kg is appropriate for intravenous glucose tolerance test (IVGTT) in miniature swine. Blood glucose levels (bG; mg/dL) were measured by glucometer at set time intervals for 120-180 minutes following rapid intravenous infusion (600 mg/kg) of glucose. Baseline bG averaged 121 in Hanford (N=2) and 74 (N=3) in Yucatan. Following intravenous infusion, bG rapidly peaked at 60 seconds increasing approx. 4-fold in Hanford and 6-fold in Yucatan and then rapidly fell back to normal levels within 30-60 minutes, with slight over compensation. Conclusion: The 600 mg/kg glucose was adequate to detect a rapid and standard IVGTT bG profiles in both miniature swine lineages. The IVGTT bG profiles are clearly different between both miniature swine lineages. The IVGTT bG profile curves for Yucatan were most homogeneous with the least variability between individuals.

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